Simple method for freezing boar spermatozoa packaged in 0.5ml plastic straws: Effects of freezing conditions and glycerol equilibration times.
نویسندگان
چکیده
منابع مشابه
Trehalose in glycerol-free freezing extender enhances post-thaw survival of boar spermatozoa
Cryopreservation of boar semen is still considered suboptimal due to lower fertility as compared with fresh samples when glycerol, a permeating cryoprotectant, is used. Trehalose is a non-permeable cryoprotectant and nonreducing disaccharide known to stabilize proteins and biologic membranes. The aim of this study was to evaluate the cryosurvival and in vitro penetrability of boar spermatozoa w...
متن کاملEffect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws.
The effects of different freezing and thawing rates on the post-thaw motility and membrane integrity of boar spermatozoa, processed as split samples in Maxi-straws or flat PET-plastic packages (FlatPack) were studied. A programmable freezing device was used to obtain freezing rates of either 20, 50 or 80 degrees C/min. Thawing of the samples was performed in a bath of circulating water; for 40s...
متن کاملEffect of rates of freezing, thawing and level of glycerol on the survival of bovine spermatozoa in straws.
Extended semen from six Hereford bulls was placed in .25-ml Continental straws and frozen in the vertical position. Treatments were arranged factorially with glycerol levels of 5, 7, 9 or 11%; semen cooled from 5 to 1 3 0 C in 3.5 (fast), 20 (moderate) or 40 min (slow); and thawed in water at 5 C for 3 min, 35 C for 12 sec, 55 C for 8 sec, 75 C for 7 sec or 90 C for 5 seconds. Fast freezing res...
متن کاملSome Factors Affecting Freezing of Boar Semen in 5 ml Maxi-straws*
A series of experiments were conducted to determine the suitable freezing and thawing temperatures for the freezing of boar semen in 5 ml maxi-straws. The ultrastructure, in vitro fertilization (IVF) and artificial insemination (AI) of frozen-thawed semen were also be evaluated. The 5 cm freezing height gave the best results not only in post-thaw motility rate (54.00%), but also in normal acros...
متن کاملP-65: Low-Density Lipoproteins Extractions on Boar Spermatozoa Quality Following Freezing–Thawing
Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...
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ژورنال
عنوان ژورنال: Nihon Yoton Gakkaishi
سال: 2002
ISSN: 1881-655X,0913-882X
DOI: 10.5938/youton.39.183